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Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  When scaling up lignocellulose-based ethanol production, the desire to increase the final ethanol titer after fermentation can introduce problems. A high concentration of water-insoluble solids (WIS) is needed in the enzymatic hydrolysis step, resulting in increased viscosity, which can cause mass and heat transfer problems because of poor mixing of the material.

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Effect of mixing on enzymatic hydrolysis of cardboard ...

Apr 01, 2012  The objectives of this study were to investigate the effect of mixing conditions on the yield of enzymatic hydrolysis and the subsequent S/L separation. The results show that mixing conditions (impeller-type, baffles and speed) do indeed have a significant effect.

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(PDF) Effect of mixing on enzymatic hydrolysis of ...

ConclusionsThe objectives of this study were to investigate the effect of mixing conditions on the yield of enzymatic hydrolysis and the subsequent S/L separation. The results show that mixing conditions (impeller-type, baffles and speed) do indeed have a significant effect.

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Mixing effects on the kinetics of enzymatic hydrolysis of ...

Dec 01, 2019  Mixing effects on the kinetics of hydrolysis. The enzymatic hydrolysis is performed in 100 ml conical flasks with cellulose concentrations of 10, 20, 30, 40 and 50 mg/ml at the optimum enzyme to substrate ratio of 1:15 (w/w) for 1 h for measuring the reaction kinetics.

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Mixing effects on the kinetics of enzymatic hydrolysis of ...

Mixing effects on enzymatic hydrolysis of cellulose extracted from Sunn hemp fibre. • Optimum enzyme:cellulose loading of 1:15 maximizes glucose and reducing sugar yields. • Kinetic constants (K m, V max, K x) decrease exponentially with mixing speed (0–150 rpm). • No mixing increases soluble sugar yield by 6–7% over continuous mixing ...

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Effect of mixing on enzymatic hydrolysis of cardboard ...

hydrolysis, mixing conditions have a considerable impact on the performance of the enzymes. Thus mixing conditions in the hydrolysis tank can also influence the downstream operations and, consequently, the overall economy of the bioethanol process. In this experimental study, four types of impeller, at different hydrolysis conditions were

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(PDF) Effect of mixing on enzymatic hydrolysis of ...

ConclusionsThe objectives of this study were to investigate the effect of mixing conditions on the yield of enzymatic hydrolysis and the subsequent S/L separation. The results show that mixing conditions (impeller-type, baffles and speed) do indeed have a significant effect.

Get Price

Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  Effect of mixing on enzymatic hydrolysis of steam-pretreated spruce: a quantitative analysis of conversion and power consumption. Benny Palmqvist, 1 Magnus Wiman, 1 and Gunnar Lidén 1. Benny Palmqvist. 1 Department of Chemical Engineering, Lund University, Box 124, Se-221 00 Lund, Sweden. Find articles by Benny Palmqvist.

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Mixing effects on the kinetics of enzymatic hydrolysis of ...

Mixing effects on enzymatic hydrolysis of cellulose extracted from Sunn hemp fibre. • Optimum enzyme:cellulose loading of 1:15 maximizes glucose and reducing sugar yields. • Kinetic constants (K m, V max, K x) decrease exponentially with mixing speed (0–150 rpm). • No mixing increases soluble sugar yield by 6–7% over continuous mixing ...

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Mixing effects on the kinetics and the dynamics of two ...

Mixing effects on the kinetics and the dynamics of two-phase enzymatic hydrolysis of hemicellulose for biofuel production. Dutta SK(1), Chakraborty S(2). Author information: (1)Department of Chemical Engineering, Indian Institute of Technology, Kharagpur 721302, India.

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Effect of enzyme and substrate dosing strategies on mixing ...

Jun 29, 2014  Enzymatic hydrolysis is one of the key steps in biofuel production from lignocellulosic material. High consistency of biomass slurry in the hydrolysis step improves the economics and energy efficiency of biofuel processes. However, it has been shown that the high consistency also decreases the hydrolysis yield and affects the rheology of hydrolysis slurry. In this study, mixing related effects ...

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Does sugar inhibition explain mixing effects in enzymatic ...

Aug 22, 2016  Enzymatic hydrolysis of lignocellulose is associated with mixing issues, which are likely to affect process performance. The aim of this study was to investigate how viscosity and sugar inhibition influence the mixing-dependence in hydrolysis of steam-pretreated spruce.

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Mixing effects on the kinetics and the dynamics of two ...

Jul 01, 2018  Mixing effects on the catalytic conversion of cellulose to biofuel products have been reported earlier (Gaikwad and Chakraborty, 2014). However, the effects of mixing – both macro- and micro-mixing – on the kinetics and the dynamics of two-phase enzymatic hydrolysis of hemicelluloses have not yet been studied.

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Effects of Enzymatic Hydrolysis of Protein on the Pasting ...

Apr 17, 2012  Abstract: As one of the most effective methods to modify proteins, enzymatic hydrolysis is used widely in the preparation of wheat products in the food industry. During the same process, starch pasting occurs frequently. The effects of wheat protein hydrolysis with papain, pepsin, and trypsin on the pasting properties of 3 different kinds of flour were investigated in 5 concentrations.

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Does sugar inhibition explain mixing effects in enzymatic ...

BACKGROUND: Enzymatic hydrolysis of lignocellulose is associated with mixing issues, which are likely to affect process performance. The aim of this study was to investigate how viscosity and sugar inhibition influence the mixing-dependence in hydrolysis of steam-pretreated spruce.

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Optimization and scale-up of enzymatic hydrolysis of wood ...

Effect of mixing mode on enzymatic hydrolysis and sugar production. (A and B) pH of manual intermittent mixing and propeller continuous mixing, respectively; (C and D) sugar production of manual intermittent mixing and propeller continuous mixing. The pulp concentration was 15%, and the enzyme dosage was 15 mg/g dry mass of each one of CMax and ...

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Effect of hemicellulose and lignin removal on enzymatic ...

Sep 01, 2007  The only significant effect on the sugar yield in the enzymatic hydrolysis that could be observed was the effect of additional xylanases and thus increased hemicellulose removal. Download : Download full-size image; Fig. 4a. Glucose yield in enzymatic hydrolysis of the Italian corn stover pretreated at 170 °C, 9 min with SO 2.

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Study On Compound Enzymatic Hydrolysis Physalis Juice And ...

In order to improve the quality of Physalis fruit juice and improve the juice suspended turbidity and color changes, the compound enzyme hydrolysis, enzyme solution conditions on the juice out of the juice yield effect; the use of high-pressure homogenization method and study the effect of the homogeneous conditions of Physalis juice grain ...

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Effects of agitation on particle-size distribution and ...

Furthermore the stirring energy dissipation may impact the particle size which in turn may affect the enzymatic hydrolysis. The objective of the current work was to specifically quantify the effects of mixing on particle-size distribution (PSD) and relate this to changes in the enzymatic hydrolysis.

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The effects of mixing on the enzymatic hydrolysis of ...

determining the effect of mixing on the enzymatic hydrolysis was the level of product inhibition. If the glucose and cellobiose concentrations were high, as during high solid hydrolysis of steam pretreated spruce, low agitation rate had a large negative effect onthe enzymatic hydrolysis.However, if the product concentration was kept low, as

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(PDF) Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading (5% WIS), decreasing the ...

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Effect of mixing on enzymatic hydrolysis of steam ...

May 11, 2011  Effect of mixing on enzymatic hydrolysis of steam-pretreated spruce: a quantitative analysis of conversion and power consumption. Benny Palmqvist, 1 Magnus Wiman, 1 and Gunnar Lidén 1. Benny Palmqvist. 1 Department of Chemical Engineering, Lund University, Box 124, Se-221 00 Lund, Sweden. Find articles by Benny Palmqvist.

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Effect of mixing on enzymatic hydrolysis of cardboard ...

The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading (5% WIS), decreasing the ...

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The effects of mixing on the enzymatic hydrolysis of ...

May 11, 2017  The effect of mixing on the enzymatic hydrolysis was in part determined by the viscosity of the pretreated biomass. For steam pretreated spruce at low solid loading, decreasing the agitation rate had little effect on the the enzymatic hydrolysis. However, if the viscosity was increased by the addition of a thickening agent, the effect of ...

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Does sugar inhibition explain mixing effects in enzymatic ...

Aug 22, 2016  Enzymatic hydrolysis of lignocellulose is associated with mixing issues, which are likely to affect process performance. The aim of this study was to investigate how viscosity and sugar inhibition influence the mixing-dependence in hydrolysis of steam-pretreated spruce.

Get Price

Optimization and scale-up of enzymatic hydrolysis of wood ...

Effect of mixing mode on enzymatic hydrolysis and sugar production. (A and B) pH of manual intermittent mixing and propeller continuous mixing, respectively; (C and D) sugar production of manual intermittent mixing and propeller continuous mixing. The pulp concentration was 15%, and the enzyme dosage was 15 mg/g dry mass of each one of CMax and ...

Get Price

Effects of Enzymatic Hydrolysis of Protein on the Pasting ...

Apr 17, 2012  Abstract: As one of the most effective methods to modify proteins, enzymatic hydrolysis is used widely in the preparation of wheat products in the food industry. During the same process, starch pasting occurs frequently. The effects of wheat protein hydrolysis with papain, pepsin, and trypsin on the pasting properties of 3 different kinds of flour were investigated in 5 concentrations.

Get Price

Effect of enzymatic high temperature prehydrolysis on the ...

Jul 14, 2015  Xylanase and mannase prehydrolysis at 80°C (2 h) improved the cellulase hydrolysis by 20–33%, while all three enzymes together improved the final enzymatic yield by 50–70%. CONCLUSION. A temperature of 100–105°C provides clear process advantages for enzymatic treatments when compared with 70–90°C.

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Effect of hemicellulose and lignin removal on enzymatic ...

Sep 01, 2007  The only significant effect on the sugar yield in the enzymatic hydrolysis that could be observed was the effect of additional xylanases and thus increased hemicellulose removal. Download : Download full-size image; Fig. 4a. Glucose yield in enzymatic hydrolysis of the Italian corn stover pretreated at 170 °C, 9 min with SO 2.

Get Price

The effect of high intensity mixing on the enzymatic ...

Dec 03, 2010  In addition, there is a synergistic effect of mixing and enzymatic hydrolysis; mixing increases the rate of cellulose conversion while the increased conversion facilitates mixing. The synergy appears to result in part from particle size reduction, which is more significant when hydrolysis occurs during intense mixing.

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Mixing Effects in Cellulase-Mediated Hydrolysis of ...

Oct 01, 2010  Conversion of cellulose to glucose is the rate-limiting step in converting biomass into fuel. In this paper, we explore the effects of micro- and macro-mixing on the enzymatic hydrolysis of various cellulosic substrates to glucose by using a spatially averaged low-dimensional CSTR model. We quantify the effects of mixing on glucose yield, cellulose depolymerization rate and the synergy

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Study On Compound Enzymatic Hydrolysis Physalis Juice And ...

In order to improve the quality of Physalis fruit juice and improve the juice suspended turbidity and color changes, the compound enzyme hydrolysis, enzyme solution conditions on the juice out of the juice yield effect; the use of high-pressure homogenization method and study the effect of the homogeneous conditions of Physalis juice grain ...

Get Price

Effects of agitation on particle-size distribution and ...

May 23, 2014  Mixing is an energy demanding process which has been previously shown to affect enzymatic hydrolysis. Concentrated biomass slurries are associated with high and non-Newtonian viscosities and mixing in these systems is a complex task. Poor mixing can lead to mass and/or heat transfer problems as well as inhomogeneous enzyme distribution, both of which can cause possible

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